Mitotic Metaphase Tie

Shown is a mitotic PtK2 cell at metaphase immunostained for microtubules (red) and kinetochores (green) with DNA stained blue. The image was obtained using structured illumination microscopy (SIM, Deltavision OMX system) which provides 'super-resolution' beyond the diffraction limit set by the wavelength of the illuminating light. The micrograph was the 2012 winner in the high- and super-resolution microscopy category of the GE Healthcare Life Sciences Imaging Contest, and featured in the NIGMS Biomedical Beat, the monthly digest of noteable NIGMS-sponsored research.